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Oxytetracycline was the second of the broad-spectrum tetracycline group of antibiotics to be discovered. Oxytetracycline works by interfering with the ability of.DNA Protocols & Applications. Preparation: Prepare LB medium according to the composition given in Liquid media. Tetracycline HCl.

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method [15]. Cells were grown aerobically in Luria Bertani (LB) medium at 37 uC with agitation. (PAO1) (gentamycin) and 200 (tetracycline) for P. aeruginosa.

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Reversal of tetracycline resistance mediated by different bacterial tetracycline resistance determinants by an inhibitor of the Tet(B) antiport protein.

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. kb, kilobase; KB, King’s B medium; Km. streptomycin; Tc, tetracycline; Tn5, transposon Tn5. overnight on LB agar or in LB broth (Sambrook.

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. (Lens culinaris Medikus subsp. culinaris) through Agrobacterium Mediated Transformation. (LB) medium and selected with 50 mg/l of tetracycline.

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Tetracycline induces transcription of tetA and. We used LB because this was the medium in which Bertrand and colleagues had characterized various properties of.